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Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml(-1). Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Gram-positive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples.
General news | 2017-12-12
See video from eminar with Professor Rashid Sumaila, one of the world’s most innovative researchers on the future of the oceans
Research news | 2017-11-30
The PECS-II conference showcased place-based research and how it can help us work towards global sustainability in the Anthropocene
Research news | 2017-11-28
How urban greening and civic ecology projects can improve human well-being and restore crucial ecosystem services
Research news | 2017-11-27
What plantain farmers in Costa Rica can teach us about the inconsistent links between access to ecosystem services and well-being
Research news | 2017-11-23
Centre science director well established among world’s most top-cited and influential scientists
Research news | 2017-11-21
Large-scale changes in Arctic marine food web can be expected within 50 years, some good, but in the long run several critical